Application Of Breeding
You can pretty much always know what you’ll get if you know what the genes are, however, it is a game of probability. Breeding a heterozygous gene with another heterozygous gives you three different gene possibilities. So, I want you to keep a few things in mind before we get into the actual explanation:
- HomoxHomo = 1 possibility
- HomoDomxHomoRess = 1 possibility
- HomoxHet = 2 possibilities
- HetxHet = 3 possibilities
These apply to all genes not including certain genetic combinations of the Agouti gene. An A/AtxAt/a or vice versa, will result in 4 possible combinations.
Let’s start simple with the Extension gene, beginning with HomoxHomo. This applies to dominant homozygous and recessive homozygous.
We have a dam with the Extension E/E and a sire with Extension E/E. If crossed, they are a “neutral”. The dominant genes have no genes to dominate, coming up with the same genes as the parents (E/E). This applies to e/e as well.
Now, if we cross a dam with the Extension e/e and a sire with Extension E/E, we can only get one combo too. Now, the Punnett Square below can explain this better than I can, but this is in the case of four different foals. Our sire’s Extension alleles are in the topmost squares. Our dam’s in the left-most. The squares below the sire and to the right of the dam each represent one foal’s Extension gene. To solve for this in one square, we must look at our dominant allele, in this case E. E will come before the dam’s e allele, resulting in an E/e gene. We continue this for each square.
Keep this in mind for later.
A homozygous and heterozygous cross will present us with two possibilities. If our dam is E/E and our sire is E/e, it will present to us this:
Half of these foals will end up Homozygous (E/E) and half heterozygous (E/e).
Heterozygous crosses will present us with:
A quarter of the foals will end up homozygous dominant, another quarter, homozygous recessive, and half heterozygous.
Now, these apply to every single gene with the exception of Agouti, particularly when it comes to this code: A/At sire and At/a dam. We have all Agouti alleles present. Our procedure however, is no different, the only thing different is the amount of possibilities. Remember, our dominant allele comes first. A (Bay) is dominant over At (Brown) and a (Black). At is dominant over a.
x | A | At |
At | AAt | AtAt |
a | Aa | Ata |
These foals will be described with their color and carrier gene: 1 foal Bay, carrying Brown. 1 foal homozygous Brown. 1 foal Bay, carrying Black. 1 foal Brown, carrying Black.
Now that we know the basics, we can apply this to the Genetics Game! I will use the genetics given to me from the game. The genetics presented have 32 possible combos.
Genes Presented:
Extension: ee Agouti: Ata Creme: CC Roan: RnRn Dun: dd Pearl: PrlPrl Silver: Zz Champagne: chch Grey: gg White: ww Tobiano: toto Splash White: splspl Sabino: SbSb Rabicano: rbrb Frame: oo Leopard Complex: lplp PATN-1: PATN-1patn-1 PATN-2: patn-2patn-2 Brindle: X- | | Extension: Ee Agouti: aa Creme: CC Roan: rnrn Dun: Dd Pearl: PrlPrl Silver: zz Champagne: chch Grey: gg White: ww Tobiano: toto Splash White: splspl Sabino: sbsb Rabicano: rbrb Frame: oo Leopard Complex: lplp PATN-1: patn-1patn-1 PATN-2: patn-2patn-2 Brindle: X- | |
Let’s not focus on the game so much, but rather getting a base line of what can be produced. I will give Punnett Squares of all changing genes.
Extension:
Agouti:
Roan:
x | Rn | Rn |
rn | Rnrn | Rnrn |
rn | Rnrn | Rnr n |
In this case, we don’t have to change this gene around, it stays Rnrn throughout the duration.
Dun:
Silver:
Sabino:
x | Sb | Sb |
sb | Sbsb | Sbsb |
sb | Sbsb | Sbsb |
This gene will also not need to be changed.
PATN-1:
x | PAT | pat |
pat | PATpat | patpat |
pat | PATpat | patpat |
We can now take a base genetic code to switch around as we need to apply this to the game. Let’s write the first foal’s genes from our Punnett Squares as our base in the form of the genetics game entry code.
Base:
Ee,Ata,CC,Rnrn,Dd,PrlPrl,Zz,chch,gg,ww,toto,splspl,Sbsb,rbrb,oo,lplp,PATN-1patn-1,patn-2patn-2
Our base is now in order, and we can begin to apply other gene switches. We’ll now copy and paste this base.
Ee,aa,CC,Rnrn,Dd,PrlPrl,Zz,chch,gg,ww,toto,splspl,Sbsb,rbrb,oo,lplp,PATN-1patn-1,patn-2patn-2
And we will switch our Agouti gene (only) with another possibility, in this case, our only option is aa. Afterward, we’ll copy the base string again, forgetting about our previous change, and change a second gene with its possibility. Our next one is Dun, with our other possibility dd. Rinse and repeat so that we only have one gene different from our base strings gene in each genetic code. You will have the following:
Ee,Ata,CC,Rnrn,dd,PrlPrl,Zz,chch,gg,ww,toto,splspl,Sbsb,rbrb,oo,lplp,PATN-1patn-1,patn-2patn-2
Ee,Ata,CC,Rnrn,Dd,PrlPrl,zz,chch,gg,ww,toto,splspl,Sbsb,rbrb,oo,lplp,PATN-1patn-1,patn-2patn-2
Ee,Ata,CC,Rnrn,Dd,PrlPrl,Zz,chch,gg,ww,toto,splspl,Sbsb,rbrb,oo,lplp,patn-1patn-1,patn-2patn-2
Next, we’ll do the same thing, except change our Extension gene, then continue along our previous pattern. You will have the following:
ee,Ata,CC,Rnrn,Dd,PrlPrl,Zz,chch,gg,ww,toto,splspl,Sbsb,rbrb,oo,lplp,PATN-1patn-1,patn-2patn-2
ee,aa,CC,Rnrn,Dd,PrlPrl,Zz,chch,gg,ww,toto,splspl,Sbsb,rbrb,oo,lplp,PATN-1patn-1,patn-2patn-2
ee,Ata,CC,Rnrn,dd,PrlPrl,Zz,chch,gg,ww,toto,splspl,Sbsb,rbrb,oo,lplp,PATN-1patn-1,patn-2patn-2
ee,Ata,CC,Rnrn,Dd,PrlPrl,zz,chch,gg,ww,toto,splspl,Sbsb,rbrb,oo,lplp,PATN-1patn-1,patn-2patn-2
ee,Ata,CC,Rnrn,Dd,PrlPrl,Zz,chch,gg,ww,toto,splspl,Sbsb,rbrb,oo,lplp,patn-1patn-1,patn-2patn-2
We’ll go back to our base string now, apply our Agouti possibility with a second, Dun, resulting in:
Ee,aa,CC,Rnrn,dd,PrlPrl,Zz,chch,gg,ww,toto,splspl,Sbsb,rbrb,oo,lplp,PATN-1patn-1,patn-2patn-2
Follow this line the same as our previous lines, starting with Silver:
Ee,aa,CC,Rnrn,dd,PrlPrl,zz,chch,gg,ww,toto,splspl,Sbsb,rbrb,oo,lplp,PATN-1patn-1,patn-2patn-2
Ee,aa,CC,Rnrn,dd,PrlPrl,Zz,chch,gg,ww,toto,splspl,Sbsb,rbrb,oo,lplp,patn-1patn-1,patn-2patn-2
Rinse and repeat with our Extension possibility:
ee,aa,CC,Rnrn,dd,PrlPrl,Zz,chch,gg,ww,toto,splspl,Sbsb,rbrb,oo,lplp,PATN-1patn-1,patn-2patn-2
ee,aa,CC,Rnrn,dd,PrlPrl,zz,chch,gg,ww,toto,splspl,Sbsb,rbrb,oo,lplp,PATN-1patn-1,patn-2patn-2
ee,aa,CC,Rnrn,dd,PrlPrl,Zz,chch,gg,ww,toto,splspl,Sbsb,rbrb,oo,lplp,patn-1patn-1,patn-2patn-2
And continue down the line with the Agouti + _:
Ee,aa,CC,Rnrn,Dd,PrlPrl,zz,chch,gg,ww,toto,splspl,Sbsb,rbrb,oo,lplp,PATN-1patn-1,patn-2patn-2
Ee,aa,CC,Rnrn,Dd,PrlPrl,zz,chch,gg,ww,toto,splspl,Sbsb,rbrb,oo,lplp,patn-1patn-1,patn-2patn-2
ee,aa,CC,Rnrn,dd,PrlPrl,Zz,chch,gg,ww,toto,splspl,Sbsb,rbrb,oo,lplp,PATN-1patn-1,patn-2patn-2
ee,aa,CC,Rnrn,dd,PrlPrl,zz,chch,gg,ww,toto,splspl,Sbsb,rbrb,oo,lplp,PATN-1patn-1,patn-2patn-2
ee,aa,CC,Rnrn,dd,PrlPrl,Zz,chch,gg,ww,toto,splspl,Sbsb,rbrb,oo,lplp,patn-1patn-1,patn-2patn-2
Ee,aa,CC,Rnrn,dd,PrlPrl,zz,chch,gg,ww,toto,splspl,Sbsb,rbrb,oo,lplp,patn-1patn-1,patn-2patn-2
ee,aa,CC,Rnrn,dd,PrlPrl,zz,chch,gg,ww,toto,splspl,Sbsb,rbrb,oo,lplp,patn-1patn-1,patn-2patn-2
Ee,Ata,CC,Rnrn,dd,PrlPrl,zz,chch,gg,ww,toto,splspl,Sbsb,rbrb,oo,lplp,PATN-1patn-1,patn-2patn-2
Ee,Ata,CC,Rnrn,dd,PrlPrl,Zz,chch,gg,ww,toto,splspl,Sbsb,rbrb,oo,lplp,patn-1patn-1,patn-2patn-2
Ee,Ata,CC,Rnrn,dd,PrlPrl,zz,chch,gg,ww,toto,splspl,Sbsb,rbrb,oo,lplp,patn-1patn-1,patn-2patn-2
ee,Ata,CC,Rnrn,dd,PrlPrl,zz,chch,gg,ww,toto,splspl,Sbsb,rbrb,oo,lplp,PATN-1patn-1,patn-2patn-2
ee,Ata,CC,Rnrn,dd,PrlPrl,Zz,chch,gg,ww,toto,splspl,Sbsb,rbrb,oo,lplp,patn-1patn-1,patn-2patn-2
ee,Ata,CC,Rnrn,dd,PrlPrl,zz,chch,gg,ww,toto,splspl,Sbsb,rbrb,oo,lplp,patn-1patn-1,patn-2patn-2
Ee,Ata,CC,Rnrn,Dd,PrlPrl,zz,chch,gg,ww,toto,splspl,Sbsb,rbrb,oo,lplp,patn-1patn-1,patn-2patn-2
ee,Ata,CC,Rnrn,Dd,PrlPrl,zz,chch,gg,ww,toto,splspl,Sbsb,rbrb,oo,lplp,patn-1patn-1,patn-2patn-2
Ee,aa,CC,Rnrn,Dd,PrlPrl,zz,chch,gg,ww,toto,splspl,Sbsb,rbrb,oo,lplp,patn-1patn-1,patn-2patn-2
ee,aa,CC,Rnrn,Dd,PrlPrl,zz,chch,gg,ww,toto,splspl,Sbsb,rbrb,oo,lplp,patn-1patn-1,patn-2patn-2
There is one extra, but I’m way too lazy to find it. But nonetheless, this is what our screens should appear as! It gets trickier the more you get into it (and the more genes to change!), but come with practice, it’ll not only get easier to understand, but easier to do!
Note: Be aware of the lines/commas on the game. It’ll mark you down!!
I will also post some Punnett Squares you guys can use for your base strings! :D